| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Megasphaera massiliensis | GCTTTGGCAAACACGAGGT | TTCCTTGCGGACGCTGTT | 59.27 | 59.89 | 203 | 122 |
100.00%
|
100.00%
|
| Primer 32 | Megasphaera massiliensis | GCTGCAAACGCTGTGTGT | CGTCTCTTCACTGCCGTCA | 59.59 | 59.71 | 245 | 121 |
100.00%
|
100.00%
|
| Primer 33 | Megasphaera massiliensis | AAGCGCATGAAACGTGGC | TCCTTGTCGACTTCGCTCA | 59.74 | 58.66 | 166 | 121 |
100.00%
|
100.00%
|
| Primer 34 | Megasphaera massiliensis | ACGTCATTGCCGTCGTCA | TTGATGCCGTTGTCGTTGC | 59.66 | 59.72 | 184 | 121 |
100.00%
|
100.00%
|
| Primer 35 | Megasphaera massiliensis | ACGTCATTGCCGTCGTCA | CGCGACATCTTCCAACAGC | 59.66 | 59.58 | 248 | 121 |
100.00%
|
100.00%
|
| Primer 36 | Megasphaera massiliensis | ACGTCATTGCCGTCGTCA | ACTGTTTAACGGCACCGGA | 59.66 | 59.55 | 297 | 121 |
100.00%
|
100.00%
|
| Primer 37 | Megasphaera massiliensis | ATTGCGCATTCCCGTTGC | CCGAGACGAGACGCTGTTT | 59.82 | 60.08 | 218 | 120 |
100.00%
|
100.00%
|
| Primer 38 | Megasphaera massiliensis | GCTGGACCAGATTCGCTGT | ACAGCGCACCTTGGACAA | 60.08 | 59.81 | 217 | 119 |
100.00%
|
100.00%
|
| Primer 39 | Megasphaera massiliensis | AGATGGAAGCACCGGAACC | CCAACGGTTCCTGCCAGAT | 59.70 | 60.00 | 264 | 119 |
100.00%
|
100.00%
|
| Primer 40 | Megasphaera massiliensis | CCGTCTAGGCCTTCGCAAT | AGGGCAGCATTCGTTGGA | 59.86 | 59.24 | 158 | 117 |
100.00%
|
100.00%
|